Coding
Part:BBa_K1620002:Design
Designed by: Celio Dias Santos Jr Group: iGEM15_UFSCar-Brasil (2015-09-03)
small heat shock protein IbpA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was made using original codon frequencies of E. coli.
Source
This part was designed from gene IbpA of E. coli K12 MG16555 genome, and synthesized by IDT. PCR amplifications were carried out using primers showed bellow and the 3A assembly method was made.
PCR primers:
Fwd: 5' - GAA TTC GCG GCC GCT TCT AGA GAT GCG TAA CTT TGA TTT ATC - 3'
Rev: 5' - TAC TAG TAG CGG CCG CTG CAG TTA GTT GAT TTC GAT ACG GC - 3'